This kit includes all the materials you will need to make thirty fruit fly cultures. The kit includes thirty 32 oz clear Twinpak cups, 15 Polyfab lids (lids are pre-punched with holes and covered with fabric- Freeze briefly between uses to avoid mite problems), and enough media to make 30 cultures. We also include excelsior wood shavings. This kit is intended for D. melanogaster flies. Kit includes no flies!!